This Operational Framework provides a practical reference toward achieving public health systems preparedness and resilience to existing and future disease threats at the human-animal-environment interface. It offers a comprehensive overview of the One Health concept and operational guidance for One Health application (what, why, and how), serving as a detailed orientation for users who wish to understand and implement this approach.

It has the following key objectives:·

• Provide operational guidance to directly address the need for targeted investments that prevent, prepare, detect, respond to, and recover from issues like diseases with endemic, emerging, and pandemic potential, including antimicrobial resistance;
• Showcase opportunities for targeting disease threats upstream (prevention at the source, or via early detection and effective response) to help reduce the frequency and impact of emergencies the system has to react to;
• Jointly yield long-term gains (and consider trade-offs) in human health, animal production, and environmental management, ultimately improving overall health of the planet and the lives, livelihoods, and well-being of people;
• Outline activities and interventions with a starting point at the human-animal-environment interface, highlight proposed methods of institutional and technical imple mentation, and enable mechanisms of coordination and partnership to build more collaborative public health systems.​​​​​​​